ELECTROPHORESIS OF PLASMA-PROTEINS

Behavior of the Serum proteins in starch-agar-gel electrophoresis

High resolution two-dimensional electrophoresis of human plasma proteins.

The two-dimensional electrophoretic technique of O'Farrell has been adapted to the analysis of human plasma proteins, and 30 polypeptides have been identified in the pattern produced. Genetic variants involving charge (isoelectric point) or size (molecular weight in the presence of sodium dodecyl sulfate) changes should be routinely detectable in at least 20 proteins at once, facilitating studi...

Electrophoresis of hydrophobic proteins

The electrophoresis of hydrophobic proteins underwent signi®cant development during the last decade. In native gel electrophoresis, the induced charge shift, the involvement of mild detergents as well as a constant ionic strength in the electrophoresis, overcame the dif®culty of the low solubility and aggregation of the hydrophobic proteins, and critically improved the results of their native P...

Gel Electrophoresis of Proteins

Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field. These techniques have become a main tool in biochemistry, molecular...

COMPARISON OF SEED PROTEINS ELECTROPHORESIS OF EIGHT SPECIES OF THE GENUS SUAEDA (CHENOPODIACEAE) IN IRAN

Seed proteins of eight species of the genus Suaeda (Chenopodiaceae) were compared by means of polyacrylamide disc gel electrophoresis. Different samples of the same species showed not less than 89% similarities, and geographical separation had no remarkable effect on seed protein patterns of the same species. Morphological heterogenity, that creates difficulties in infrageneric classificat...